No methodology has been built to distinguish intracellular SERS nanosensors from cell outer membrane bound-ones. Here we propose a “turning off” strategy by combining etchable SERS nanosensors with a non-permeable etchant. The SERS signals outside the living cells can be rapidly removed, leaving only the internalized nanosensors for imaging and thereby allowing accurate intracellular pH and H2O2 sensing.
The 20th Chinese NationalConference on Light Scattering organized by Suzhou University and Xiamen Universitywas held in Suzhou from Nov. 2-6, 2019.Lu Bai was awarded the Best Poster Award. Congratulations!